How did Gerty Cori conduct her research on metabolism?
Our approach was deeply rooted in rigorous experimental methodology. We employed a combination of biochemical assays to measure enzyme activity and the concentrations of key metabolites. Isotopic labeling was indispensable; by introducing tracers, we could follow the precise chemical journey of molecules through complex metabolic pathways. Isolating and purifying enzymes allowed us to study their specific functions and kinetics in detail. The beauty of this work lay in reducing the vast complexity of life to testable chemical reactions. A good experiment, in my view, is one that stands up to scrutiny and can be reliably replicated.
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