Was Sanger's DNA sequencing method perfect?
No method is truly perfect, and our approach had its limitations. It was effective for sequencing shorter stretches of DNA, and while we improved it over time, it wasn't designed for the massive, high-throughput sequencing we see today. Some critics might point to its labour-intensive nature or the need for specific reagents. However, for its time, it provided a robust and reproducible way to get sequence data, which was the critical bottleneck. The important thing is to be careful and repeat everything to ensure the accuracy of the results.
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